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Kandere-Grzybowska, Kristiana Telephone : 052-217-5516

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    Kristiana Kandere-Grzybowska, Ph.D.
    Senior Research Fellow, IBS
    Adjunct Professor, UNIST

    Biography 

    Kristiana Kandere-Grzybowska graduated summa cum laude in biology from the College of Saint Rose (Albany, NY, USA) in 1998. She obtained a doctoral degree in biochemistry from Tufts University School of Medicine (Boston, MA, USA) in September 2003 (with T.C. Theoharides). From 2003-2006 she was a Department of Defense (DOD) postdoctoral fellow (with G. G. Borisy) at Northwestern University (Chicago, Illinois, USA). From 2006-2014 she was a Research Assistant Professor in the Department of Chemical and Biological Engineering at Northwestern University and worked with Prof. Bartosz A. Grzybowski. She joined IBS Center for Soft and Living Matter in 2015.


    Scientific Interests:
    molecular dynamic live cell imaging, cell and cytoskeleton micropatterning via micro/nano-fabrication; intracellular/cytoskeleton dynamics, and cancer cell motility; interactions of nanomaterials with biological systems, mixed-charge nanomaterials with cancer-specific cytotoxicity.



    Highlights of Scientific Contributions: 
    Kandere-Grzybowska pioneered the microetching method (also called Wet Stamping, or WETS) for cell micropatterning that is compatible with various high-resolution live-cell imaging modalities, such as wide-field digital fluorescence, confocal and total internal reflection (TIRF) microscopies (Nature Methods, 2005). She and colleagues then used WETS to study intracellular cell motility sub-processes, such as cell polarization (Soft Matter, 2010), microtubule guidance (J. Cell Sci., 2012), and cell micromechanics (Adv. Mater., 2012) in micropatterned cells with geometrically-defined shapes (also called Treadmills). WETS were also used to demonstrate that asymmetric continuous ratchet-shape patterns (Ratchets) direct cell motions. Specifically, cancer cells' motions were directed in the direction opposite to that by normal cells leading to partial sorting out of mixed cell populations (Nature Physics, 2009). Finally, using continuous linear patterns (or Lines or 1D microtracks) generated by WETS revealed that metastatic cells, but not non-metastatic cells, use cell-intrinsic, predator-like Lévy walk patterns (Nature Communications, 2018).  Kandere-Grzybowska is an author of 34 publications cited 3020 times (based on Google Scholar) with an h-index 23.

     

    Her more recent work focuses on understanding the cellular mechanisms of cancer-specific, selective cytotoxicity of mixed-charge gold nanoparticles (Angew. Chem., 2016). A combination of chemistry (nanoparticle synthesis/functionalization), nanomaterial characterization (DLS, ICP-MS), and cell biological approaches (live-cell confocal microscopy, bio-TEM, proteomics) are used to investigate selective targeting and destruction of the endolysosomal system in cancer cells (Nat. Nanotechnol.,2020; Acc. Mater. Res, 2020).

     

    Recent publications:

     

    Siek, M., Kandere-Grzybowska, K.* & Grzybowski, B.A.* Mixed-Charge, pH-Responsive nanoparticles for selective interactions with cells, organelles, and bacteria. [Review] Acc. Mater. Res. 1, 3, 188–200 (2020). https://doi.org/10.1021/accountsmr.0c00041

     

    Borkowska, M., Siek, M., Kolygina, D., Sobolev, Y., Lach, S., Kumar, S., Cho, Y.K, Kandere-Grzybowska, K.* & Grzybowski, B.A.* Targeted crystallization of mixed-charge nanoparticles in lysosomes induces selective death of cancer cells. Nat. Nanotechnol. 15, 331–341 (2020). https://doi.org/10.1038/s41565-020-0643-3  [COVER ART] https://rdcu.be/b21hN 

     

    Huda, S., Weigelin, B., Wolf, K., Tretiakov, K., Wilk, G., Iwasa, M., Banaszak, M., Soh, S., Makurath, M., Friedl, P., Borisy, G.G., Kandere-Grzybowska, K.* & Grzybowski, B.A.*. Lévy-like movement patterns of metastatic cancer cells revealed in microfabricated systems and implicated in vivo. Nat. Commun. 9, 4539 (2018). https://rdcu.be/barxd

     

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